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comGA [2020-01-26 12:40:15]
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comGA [2020-01-26 12:40:15]

late competence protein, traffic ATPase, binds and transports transforming DNA, required for growth arrest of competent cells
Locus
BSU_24730
Isoelectric point
8.89
Molecular weight
40.30 kDa
Protein length
356 aa Sequence Blast
Gene length
1071 bp Sequence Blast
Function
genetic competence
Product
traffic ATPase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,559,007 2,560,077

Phenotypes of a mutant

The protein

Catalyzed reaction/ biological activity

  • binds and transports transforming DNA
  • sequesters MreB during expression of [Pubmed|genetic competence] to inhibit cell elongation during escape from competence PubMed
  • Protein family

  • GSP E family (single member, according to UniProt)
  • Modification

  • phosphorylated on Arg-28, Arg-71- and Arg-330 PubMed
  • Structure

  • 4PHT (from Vibrio vulnificus, 38% identity) PubMed
  • Localization

  • peripheral membrane protein PubMed
  • localizes to cell poles in competent cells PubMed
  • localizes to one cell pole PubMed
  • Additional information

  • ComGA stability depends on Maf PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigF: sigma factor, PubMed, in SigF regulon
  • Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed
  • Additional information

  • the comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL operon requires NusA for expression Reference
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    Biological materials

    Mutant

  • BKE24730 (comGA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTGATTCCCTCTCCTT, downstream forward: _UP4_TAGAAAAGTCTGGTTGTTAA
  • BKK24730 (comGA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTGATTCCCTCTCCTT, downstream forward: _UP4_TAGAAAAGTCTGGTTGTTAA
  • BP914 ((comGA-comGB-comGC-comGD-comGE-comGF-comGG)::phleo), available in Fabian Commichau's lab
  • GFP fusion

  • GP1640 trpC2 ymdB::spc PcomG-gfp (cat), available in Jörg Stülke's lab
  • GP1643 PcomG-gfp (cat) in the NCIB3610 background, available in Jörg Stülke's lab
  • PG389 amyE::(pcomGA-lacZ gfp cat) PubMed, available in Leendert Hamoen's and Jörg Stülke's labs
  • References

    Reviews

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    Original publications

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